18–20 July, 2017Max 16 people, lectures and practical course
Max 50 people, lectures only
Poster for download is HERE.
Molecular biology has undergone a revolution, with advances in molecular cloning, synthetic biology, and genome engineering allowing researchers to perform experiments that were only dreamed of a few years ago. This workshop will provide an overview of recent advances in molecular cloning technologies, with a particular focus on recombination-based cloning and CRISPR/Cas9 genome engineering.
The practical component of the workshop will focus on genome editing of a MEF cell line expressing GFP, and introduce participants to the use of electroporation of RNA-protein complexes (RNPs), gRNA design principles and identification of edited clones, and SLIC/Golden Gate design principles.
The practical work will be accompanied by lectures on SLIC, Gibson, and Golden Gate cloning technologies, CRISPR/Cas9 in mammalian systems and plants, multi-gene cloning and its appl ications, and in vivo applications of CRISPR/Cas9.
Lectures will be given by VBCF-ProTech staff:
and selected guest lecturers:
Ross Kettleborough (Twist Bioscience)
JULY 18, Day 1
| 9:00–12:30 | Start of practical course |
|---|---|
| 12:30–13:30 | Lunch break |
| 13:30–14:45 | Introduction to Modern Molecular Cloning Methods |
| 14:45–15:00 | Coffee break |
| 15:00–16:00 | Introduction to Genome Engineering with CRISPR/Cas9 |
JULY 19, Day 2
| 9:00–9:45 | Applications of SLIC and Golden Gate cloning to protein expression |
|---|---|
| 9:45–10:30 | Guest lecture - Florian Weissmann |
| 10:30–10:45 | Coffee break |
| 10:45–11:30 | Applications of CRISPR/Cas9 in mammalian systems |
| 11:30–12:15 | CRISPR/Cas9 and Modern cloning techniques in plants |
| 12:15–13:00 | Lunch break |
| 13:00–17:00 | Practical course |
JULY 20, Day 3
| 9:00–9:45 | Guest lecture – Ross Kettleborough |
|---|---|
| 9:45–10:45 | Guest lecture - Ralf Kuehn |
| 10:45–11:00 | Coffee break |
| 11:00–12:15 | Practical Course Wrap-up |
| End of course |
