The main focus of the group is researching the structural aspects of the integrity and stability of globular domains truncated by alternative splicing. We are developing a web server that uses the principles of protein disorder and exposed hydrophobic surface to predict the chances of survival for such domains and, by proxy, the alternatively spliced proteins containing such domains.
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Biomolecular Interactions & Crystallization
The core facility provides services leading to structure characterization of biomolecules and to study (bio)molecular interactions in a real time using mainly biosensor and calorimetry-based methods. It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, CD spectroscopy, analytical ultracentrifugation).
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Core Facility Cellular Imaging (CELLIM) provides services in the area of light microscopy and offers comprehensive expertize related to sample preparation, image acquisition and data analysis. We also provide access to widefiled and confocal microscopes and individual training of users in operation of microscopes, image acquisition and data analysis.
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Cryo-electron Micsroscopy & Tomography
Modern electron microscopy in structural biology on the cellular and molecular level is performed by cryo-electron microscopy and cryo-electron tomography. Cryo-electron tomography (cryo-ET) is the only method to address pleiomorphic structures like cells and organelles in a close to native state, while cryo-electron microscopy (cryo-EM) is applied to study single particles, primarily larger macromolecular complexes, which have been isolated and purified by biochemical methods. Both methodologies provide information on the cellular and molecular level and are therefore ideal for in-depth structural-functional analysis in combination with state of the art biochemical characterisation. The main activities of the Core Facility will be centred on application of cryo-EM and cryo-ET, implementations of the required image processing capabilities, and explorations of suitable cryo-preparation techniques.
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The Core Facility offers services and access to the instrument for genomic analyses. Equipment for massively parallel (Next Generation) sequencing, DNA microarrays as well as capillary sequencing and real-time qPCR (including high-throughput and digital PCR) is available or will be available soon. Services: Nextera and TruSeq compatible NGS library preparation including precise small samples, capillary sequencing, gene expression microarrays, CGH and cytogenetics microarrays.
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Multimodal and Functional Imaging Laboratory
MAFIL is open CEITEC laboratory providing advanced imaging services for CEITEC research teams as well as external users. Basic techniques on which the action is based are human MR imaging and MR spectroscopy in high field (3 T) and electrophysiological techniques (high density EEG, simultaneous measurements of electrophysiology and MRI). MAFIL expertise is mainly focused on functional and structural mapping of human brain because this core-facility was designed primarily to support neuroscience research. Regarding the fact CF MAFIL performs measurements of living subjects access to laboratory services conforms to specific organizational and operational guidelines.
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Plant Sciences Core Facility is an open CEITEC laboratory providing access to the research infrastructure for basic plant growth, environmental simulation and phenotyping analyses. The core facility operates 15 fully controlled walk-in growth chambers (phytotrons) and 10 greenhouses to provide defined environment for controlled plant growing. It serves as a focal point for broad-based cutting-edge biological research.
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