V
BCF will host a hands-on workshop on Exome library preparation presented by our colleagues from the CEITEC Genomics group.
When: Tuesday 31.07 to Thursday 02.08. 2018
Where: VBCF building, VBC2 1st floor
Registering is necessary, please write to solexa.service@imp.ac.at if you would like to participate.
A maximum of 12 attendants will be accepted, slots will be given away on a first comes first serve policy.
Reagents for the full procedure will be provided by the facility and will be funded by an interregional Czech-Austria collaborative grant.
Programme:
Day 1:
9:00–10:00: Introduction
10:00–11:00: Set up Fragmentation and Endrepair
11:00–12:00: Presentation: DNA library preparation methods
12:30–13:00: Lunch
13:00–16:00: Adaptor Ligation and PCR, check library on Bioanalyzer
Day 2:
9:00–~11:00: set up hybridization
11:00–12:00: Presentation1: Enrichment strategies (Hybridization, PCR)
12:00–13:00: Presentation 2: Data processing
Note: no lunch break, but a coffee break
Day 3:
9:00–12:30: Wash and Recover the Captured DNA sample
12:30–13:00: Lunch
13:00–15:00: Post Capture PCR Amplification
(during PCR short talk about NEB products)
Purification
15:00–16:00: Quality control of finished samples using the Bioanalyzer. Final discussion and closing of the workshop
Coffee breaks and lunch for workshop participants included
