Modern Techniques in Molecular Biology and Genome Engineering

18–20 July, 2017

Vienna Biocenter, Dr. Bohr-Gasse 3

Max 16 people, lectures and practical course

Max 50 people, lectures only

Poster for download is HERE.

General workshop info

Molecular biology has undergone a revolution, with advances in molecular cloning, synthetic biology, and genome engineering allowing researchers to perform experiments that were only dreamed of a few years ago.  This workshop will provide an overview of recent advances in molecular cloning technologies, with a particular focus on recombination-based cloning and CRISPR/Cas9 genome engineering.

The practical component of the workshop will focus on genome editing of a MEF cell line expressing GFP, and introduce participants to the use of electroporation of RNA-protein complexes (RNPs), gRNA design principles and identification of edited clones, and SLIC/Golden Gate design principles. 

The practical work will be accompanied by lectures on SLIC, Gibson, and Golden Gate cloning technologies, CRISPR/Cas9 in mammalian systems and plants, multi-gene cloning and its appl ications, and in vivo applications of CRISPR/Cas9.

Lectures will be given by VBCF-ProTech staff:

  • Krzysztof Chylinski
  • Jana Neuhold
  • Vera Schoft
  • Peggy Stolt-Bergner

and selected guest lecturers:

  • Florian Weissmann (IMP Vienna)
  • Ralf Kühn (MDC Berlin)
  • Ross Kettleborough (Twist Bioscience)


Programme

JULY 18, Day 1

9:00–12:30Start of practical course
12:30–13:30Lunch break
13:30–14:45Introduction to Modern Molecular Cloning Methods
14:45–15:00Coffee break
15:00–16:00

Introduction to Genome Engineering with CRISPR/Cas9


JULY 19, Day 2

9:00–9:45Applications of SLIC and Golden Gate cloning to protein expression
9:45–10:30Guest lecture - Florian Weissmann
10:30–10:45Coffee break
10:45–11:30Applications of CRISPR/Cas9 in mammalian systems
11:30–12:15

CRISPR/Cas9 and Modern cloning techniques in plants

12:15–13:00Lunch break
13:00–17:00Practical course


JULY 20, Day 3

9:00–9:45Guest lecture – Ross Kettleborough
9:45–10:45Guest lecture - Ralf Kuehn
10:45–11:00Coffee break
11:00–12:15Practical Course Wrap-up

End of course