Max 16 people, lectures and practical course
Max 50 people, lectures only
Poster for download is HERE.
Molecular biology has undergone a revolution, with advances in molecular cloning, synthetic biology, and genome engineering allowing researchers to perform experiments that were only dreamed of a few years ago. This workshop will provide an overview of recent advances in molecular cloning technologies, with a particular focus on recombination-based cloning and CRISPR/Cas9 genome engineering.
The practical component of the workshop will focus on genome editing of a MEF cell line expressing GFP, and introduce participants to the use of electroporation of RNA-protein complexes (RNPs), gRNA design principles and identification of edited clones, and SLIC/Golden Gate design principles.
The practical work will be accompanied by lectures on SLIC, Gibson, and Golden Gate cloning technologies, CRISPR/Cas9 in mammalian systems and plants, multi-gene cloning and its appl ications, and in vivo applications of CRISPR/Cas9.
Lectures will be given by VBCF-ProTech staff:
and selected guest lecturers:
Ross Kettleborough (Twist Bioscience)
JULY 18, Day 1
|9:00–12:30||Start of practical course|
|13:30–14:45||Introduction to Modern Molecular Cloning Methods|
Introduction to Genome Engineering with CRISPR/Cas9
JULY 19, Day 2
|9:00–9:45||Applications of SLIC and Golden Gate cloning to protein expression|
|9:45–10:30||Guest lecture - Florian Weissmann|
|10:45–11:30||Applications of CRISPR/Cas9 in mammalian systems|
CRISPR/Cas9 and Modern cloning techniques in plants
JULY 20, Day 3
|9:00–9:45||Guest lecture – Ross Kettleborough|
|9:45–10:45||Guest lecture - Ralf Kuehn|
|11:00–12:15||Practical Course Wrap-up|
|End of course|